女性护理液抗炎止痒作用的实验研究

女性护理液为中药苦参醇提液加三氯羟基二苯醚复配而成，根据药理方法学以实验动物建立模型，观察护理液的抗炎、止痒作用。结果，单独使用三氯羟基二苯醚，有较好的抗菌消炎作用，但是止痒作用不明显。以苦参醇提液和三氯羟基二苯醚复配，其抗炎、止痒作用均接近地塞米松磷酸钠注射液。     

正交实验优选槐花多糖的最佳提取工艺及抑菌活性研究

优选槐花多糖的提取工艺和含量,采用水提法和正交实验法研究了溶媒比、浸提温度和浸提时间3因素对槐花中多糖提取的影响,筛选出优化水平组合:料液比为1:15、浸提温度是95℃、浸提时间是6h,槐花多糖的含量为0.0626g/g,且对金黄葡萄球菌、大肠杆菌、枯草芽孢杆菌均有一定的抑菌活性,结果可为进一步研究开发提供依据。     

微波辅助提取苦参总黄酮

研究苦参中黄酮类化合物的微波提取最佳工艺。采用单因子及正交实验，考察了乙醇浓度、微波时间、微波功率及固液比对苦参黄酮类化合物提取量的影响规律。结果：苦参黄酮类化合物提取工艺的最佳参数组合为：乙醇体积分数10％，微波作用时间6min，功率为420w，固液比为1：25（g／mL）。在最佳参数组合下苦参中总黄酮的提取量可达4．83％。结论：采用微波技术提取苦参中总黄酮具有良好的应用前景。     

Importance of biotic interactions in large rivers: an experiment with planktivorous fish,dreissenid mussels and zooplankton in the St. Lawrence River

Physical conditions are usually considered pre‐eminent in controlling river plankton, but biotic interactions may be important in slackwater areas. To begin testing this general hypothesis, we conducted a 12‐day, predator–prey experiment in 3500 litre mesh enclosures in a slackwater area of the St. Lawrence River using planktivorous, juvenile yellow perch (Perca flavescens) and Dreissena mussels. Results generally supported our hypotheses that: (1) perch would directly suppress large zooplankton via predation but benefit microzooplankton through indirect interactions; and (2) dreissenids would directly depress rotifer densities via predation and have indirect negative effects on macrozooplankton. Based on gut contents of experimental fish, cladocera were the principal prey of smaller yellow perch (c. 46–50 mm), followed by copepods, ostracods, and rotifers. Larger juvenile perch (c. 67–73 mm) fed almost exclusively on copepods and ostracods. Densities of calanoid copepodids, nauplii, and some cyclopoid copepods (Diacyclops thomasi) were significantly depressed by perch, and adult Eurytemora affinis (99% of adult calanoids) essentially disappeared from fish enclosures. Despite being a favourite prey item of small perch, densities of the small cladoceran Bosmina (Sinobosmina) spp. were significantly higher when fish were present (150% greater than control densities on Day 12). Densities of the very abundant rotifer Polyarthra were >300% greater in fish enclosures than controls by Day 12, and the rotifers Synchaeta and predaceous Ploesoma were significantly more abundant in the presence of perch. Increases in rotifers and cladocera suggest indirect, positive effects of fish related to significantly higher phytoplankton biomass or decreased densities of predaceous copepods. Densities of eight of ten zooplankton groups examined declined significantly when mussels were present, and calanoid copepodids also declined but not significantly. Chlorophyll‐a concentrations were slightly lower in mussel enclosures. This evidence suggests biotic interactions play important roles among potamoplankton in slackwater habitats, but river‐wide implications of these findings require further study. Copyright © 2003 John Wiley & Sons, Ltd.     

4种药剂对甘草小绿叶蝉的防效评价

研究了几种药剂对甘草小绿叶蝉的防效.几种供试药剂对甘草小绿叶蝉都表现出较高的防效.2.0%阿维菌素EC施药后7d的各质量浓度虫口减退率都高于80%,持效性较好.     

槐米提取液的抗氧化性能及在乳液化妆品中的应用研究

考察槐米提取液的体外抗氧化、美白能力及加入乳液化妆品后的保湿、美白功效。实验结果表明:槐米提取液具有一定的体外抗氧化活性,与传统的抗氧化产品V_C相比,其对DPPH的清除效果好于V_C,对羟基自由基的清除率以及还原能力与V_C接近;0.3 g/L的槐米提取液对酪氨酸酶的抑制率为60.33%;添加槐米提取液的乳液化妆品能有效提高其保湿性,增强其美白功效,对皮肤安全、无刺激。     

Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.     

苦参愈伤组织诱导及继代培养条件研究

目的：研究苦参愈伤组织诱导与继代培养,为药用成分苦参碱及氧化苦参碱的来源提供新途径.方法：考察了不同外植体和激素组合对苦参愈伤组织诱导的影响和苦参愈伤组织增殖继代的最佳培养条件.结果：分别以幼芽、根、茎段、叶片为外植体,在MS＋1.0mg/L 2,4-D＋1.0mg/L 6-BA＋1.0mg/L NAA激素组合下苦参愈伤组织的诱导率分别为94.12%、74.19%、35.71%、23.08%;在MS＋0.5mg/L 2,4-D＋0.1mg/L 6-BA＋0.5mg/L NAA激素组合下,苦参愈伤组织增殖率最高,为25.3%.结论：幼芽为苦参愈伤组织诱导最佳外植体.不同激素组合对愈伤组织诱导率的影响不大,但对其增殖的影响较大,其中NAA具有显著影响.